10.13130/RD_UNIMI/OXXJ1NBattaglia, CristinaCristinaBattaglia0000-0003-3025-9657University of MilanUNIMI Dataverse2022Medicine, Health and Life Sciencescellular senescence, Noncoding RNA, Long, lncRNA, Untranslated RNA, Real-Time PCRBattaglia, CristinaCristinaBattagliaUniversity of MilanMarco VenturinMarcoVenturinCristina BattagliaCristinaBattaglia2021-09-122021-12-292022-01-05aggregate data19893311626792application/pdfapplication/pdf1.0CC0 WaiverCellular senescence is a hallmark of aging and is the result of a variety of stresses, such as telomere attrition, DNA damage and mitochondrial dysfunction. Moreover, senescent cells display a typical secretory phenotype, which involves the secretion of several inflammatory factors, as well as increased levels of beta-galactosidase activity in the lysosomes. Senescent cells accumulate during aging and have been implicated in promoting a variety of age-related diseases, including neurodegenerative diseases. Autophagy, the degradation system whereby the cells recycle dysfunctional proteins and damaged organelles, is one of the processes affected by senescence, but it is also able to induce senescence. Among the different mechanisms controlling the senescence status and the autophagic process, a key role of non-coding RNAs (ncRNAs), both miRNAs and long non-coding RNAs (lncRNAs), is now clearly emerging. AIM OF THE PROJECT With the aim to explore a possible dysregulation of ncRNAs in senescence, we will measured by qPCR the levels of a set of miRNAs and lncRNAs associated to autophagy, senescence and aging in different cellular models of replicative and chemical induced senescence. SUPPORTED BY The investigation was supported by PSD2020, University of Milan to Cristina Battaglia and Marco Venturin.Milan, ItalyUniversity of MilanPSD-2020