Raw data of 16S rRNA gene sequencinghttps://doi.org/10.13130/RD_UNIMI/THVHPOMantegazza, GiacomoUNIMI Dataverse2022-12-162023-12-13T11:09:26ZThis dataset contains the following files: 126 files \"fastq.gz\": files containing the raw sequencing data (FASTQ files) of the 16S rRNA gene profiling carried out on DNA extracted from rocket salad cultivated with vertical farming techniques (from A1 to L3) and rocket salad cultivated with horizontal farming techniques (from Rock03001 to Rock05013). The 16S rRNA gene was amplified using primers 341F: 5'-CCTACGGGNGGCWGCAG-3' and 805R: 5'-GACTACHVGGGTATCTAATCC-3’ (DOI: 10.1093/nar/gks808), which target the V3-V4 hypervariable regions, and sequenced using MiSeq Illumina® technology (2 x 300 paired-end MiSeq kit V3, set to encompass 467-bp amplicon) as previously described (DOI: 10.1371/journal.pone.0142334; DOI: 10.1111/trf.13477). For any additional need to this datasets, contact: giacomo.mantegazza@unimi.it and simone.guglielmetti@unimi.it.Medicine, Health and Life Sciences2022-12-16Mantegazza, Giacomo2022-02-18CC0 1.0