Deliverable D2.1: Linear and cyclic peptide aptamer libraries (ICPSR doi:10.13130/RD_UNIMI/R2OS1K)

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Part 1: Document Description
Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Part 5: Other Study-Related Materials
Entire Codebook

Document Description

Citation

Title:

Deliverable D2.1: Linear and cyclic peptide aptamer libraries

Identification Number:

doi:10.13130/RD_UNIMI/R2OS1K

Distributor:

UNIMI Dataverse

Date of Distribution:

2020-01-04

Version:

2

Bibliographic Citation:

Pesaresi, Paolo, 2020, "Deliverable D2.1: Linear and cyclic peptide aptamer libraries", https://doi.org/10.13130/RD_UNIMI/R2OS1K, UNIMI Dataverse, V2, UNF:6:fu9U/UKdlliZqAI94tsvXQ== [fileUNF]

Study Description

Citation

Title:

Deliverable D2.1: Linear and cyclic peptide aptamer libraries

Subtitle:

Sequences of the libraries to verify their quality

Identification Number:

doi:10.13130/RD_UNIMI/R2OS1K

Authoring Entity:

Pesaresi, Paolo (University of Milano)

Distributor:

UNIMI Dataverse

Date of Distribution:

2020-01-04

Study Scope

Keywords:

Agricultural Sciences, Aptamer

Abstract:

Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).

Methodology and Processing

Data Access

File Description--f3556

File: Sequences from generated Y2H peptide libraries.tab

  • Number of cases: 102

  • No. of variables per record: 7

  • Type of File: text/tab-separated-values

Notes:

UNF:6:fu9U/UKdlliZqAI94tsvXQ==

Variable Description

List of Variables:

Variables

LinearPeptideLibrary(LPL)

f3556 Location:

Variable Format: character

Notes: UNF:6:gSkXMCzq44SHDLK29ZHIVg==

B

f3556 Location:

Variable Format: character

Notes: UNF:6:QAdQUr9bIrH7HfXCzrTyMA==

C

f3556 Location:

Variable Format: character

Notes: UNF:6:sZ01393Gqh+0F0Y1ZVVd4A==

D

f3556 Location:

Variable Format: character

Notes: UNF:6:IUEcbH1/SOJ1WuY4XivmyA==

E

f3556 Location:

Summary Statistics: StDev NaN; Min. NaN; Valid 0.0; Max. NaN; Mean NaN

Variable Format: numeric

Notes: UNF:6:LNa/Wr4lYd7bydCOSfH8uA==

F

f3556 Location:

Summary Statistics: Valid 0.0; Max. NaN; Min. NaN; Mean NaN; StDev NaN

Variable Format: numeric

Notes: UNF:6:LNa/Wr4lYd7bydCOSfH8uA==

G

f3556 Location:

Summary Statistics: StDev NaN; Mean NaN; Min. NaN; Valid 0.0; Max. NaN

Variable Format: numeric

Notes: UNF:6:LNa/Wr4lYd7bydCOSfH8uA==

Other Study-Related Materials

Label:

Deliverable_D2.1.pdf

Text:

Description of the strategies used to generate the aptamer libraries

Notes:

application/pdf