Deliverable D2.1: Linear and cyclic peptide aptamer librariesdoi:10.13130/RD_UNIMI/R2OS1KUNIMI Dataverse2020-01-042Pesaresi, Paolo, 2020, "Deliverable D2.1: Linear and cyclic peptide aptamer libraries", https://doi.org/10.13130/RD_UNIMI/R2OS1K, UNIMI Dataverse, V2, UNF:6:fu9U/UKdlliZqAI94tsvXQ== [fileUNF]Deliverable D2.1: Linear and cyclic peptide aptamer librariesSequences of the libraries to verify their qualitydoi:10.13130/RD_UNIMI/R2OS1KPesaresi, Paolo828940UNIMI DataversePesaresi, PaoloPesaresi, Paolo2020-01-03Agricultural SciencesAptamerDescription of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).Restricted to the NoPEST partners.Restricted to the members of NoPEST consortiumSequences from generated Y2H peptide libraries.tab1027text/tab-separated-valuesUNF:6:fu9U/UKdlliZqAI94tsvXQ==Deliverable_D2.1.pdfDescription of the strategies used to generate the aptamer librariesapplication/pdf