Deliverable D2.1: Linear and cyclic peptide aptamer librarieshttps://doi.org/10.13130/RD_UNIMI/R2OS1KPesaresi, PaoloUNIMI Dataverse2020-01-042024-01-30T15:41:24ZDescription of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).Agricultural SciencesAptamer2020-01-04Pesaresi, Paolo2020-01-03Restricted to the NoPEST partners.