{"@context":"http://schema.org","@type":"Dataset","@id":"https://doi.org/10.13130/RD_UNIMI/R2OS1K","identifier":"https://doi.org/10.13130/RD_UNIMI/R2OS1K","name":"Deliverable D2.1: Linear and cyclic peptide aptamer libraries","creator":[{"@type":"Person","givenName":"Paolo","familyName":"Pesaresi","affiliation":{"@type":"Organization","name":"University of Milano"},"name":"Pesaresi, Paolo"}],"author":[{"@type":"Person","givenName":"Paolo","familyName":"Pesaresi","affiliation":{"@type":"Organization","name":"University of Milano"},"name":"Pesaresi, Paolo"}],"datePublished":"2020-01-04","dateModified":"2024-01-30","version":"2","description":"Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).","keywords":["Agricultural Sciences","Aptamer"],"license":"https://dataverse.unimi.it/api/datasets/:persistentId/versions/2.1/customlicense?persistentId=doi:10.13130/RD_UNIMI/R2OS1K","includedInDataCatalog":{"@type":"DataCatalog","name":"UNIMI Dataverse","url":"https://dataverse.unimi.it"},"publisher":{"@type":"Organization","name":"UNIMI Dataverse"},"provider":{"@type":"Organization","name":"UNIMI Dataverse"},"funder":[{"@type":"Organization","name":"European Commission"}],"distribution":[{"@type":"DataDownload","name":"Deliverable_D2.1.pdf","encodingFormat":"application/pdf","contentSize":1450282,"description":"Description of the strategies used to generate the aptamer libraries","@id":"https://doi.org/10.13130/RD_UNIMI/R2OS1K/FQ45ZF","identifier":"https://doi.org/10.13130/RD_UNIMI/R2OS1K/FQ45ZF","contentUrl":"https://dataverse.unimi.it/api/access/datafile/3544"},{"@type":"DataDownload","name":"Sequences from generated Y2H peptide libraries.tab","encodingFormat":"text/tab-separated-values","contentSize":5375,"description":"200 sequences from linear and cyclic peptide libraries to test their quality","@id":"https://doi.org/10.13130/RD_UNIMI/R2OS1K/HLSOQT","identifier":"https://doi.org/10.13130/RD_UNIMI/R2OS1K/HLSOQT","contentUrl":"https://dataverse.unimi.it/api/access/datafile/3556"}]}