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Part 1: Document Description
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Citation |
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Title: |
Deliverable D2.1: Linear and cyclic peptide aptamer libraries |
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Identification Number: |
doi:10.13130/RD_UNIMI/R2OS1K |
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Distributor: |
UNIMI Dataverse |
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Date of Distribution: |
2020-01-04 |
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Version: |
2 |
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Bibliographic Citation: |
Pesaresi, Paolo, 2020, "Deliverable D2.1: Linear and cyclic peptide aptamer libraries", https://doi.org/10.13130/RD_UNIMI/R2OS1K, UNIMI Dataverse, V2, UNF:6:fu9U/UKdlliZqAI94tsvXQ== [fileUNF] |
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Citation |
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Title: |
Deliverable D2.1: Linear and cyclic peptide aptamer libraries |
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Subtitle: |
Sequences of the libraries to verify their quality |
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Identification Number: |
doi:10.13130/RD_UNIMI/R2OS1K |
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Authoring Entity: |
Pesaresi, Paolo (University of Milano) |
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Grant Number: |
828940 |
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Distributor: |
UNIMI Dataverse |
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Access Authority: |
Pesaresi, Paolo |
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Depositor: |
Pesaresi, Paolo |
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Date of Deposit: |
2020-01-03 |
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Holdings Information: |
https://doi.org/10.13130/RD_UNIMI/R2OS1K |
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Study Scope |
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Keywords: |
Agricultural Sciences, Aptamer |
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Abstract: |
Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs). |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Notes: |
Restricted to the NoPEST partners. |
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Restricted to the members of NoPEST consortium |
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Other Study Description Materials |
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File Description--f3556 |
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File: Sequences from generated Y2H peptide libraries.tab |
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Notes: |
UNF:6:fu9U/UKdlliZqAI94tsvXQ== |
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Label: |
Deliverable_D2.1.pdf |
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Text: |
Description of the strategies used to generate the aptamer libraries |
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Notes: |
application/pdf |