10.13130/RD_UNIMI/R2OS1K
Pesaresi, Paolohttps://orcid.org/0000-0002-3236-7005(University of Milano)
Deliverable D2.1: Linear and cyclic peptide aptamer libraries
UNIMI Dataverse
2020
doi:10.13130/RD_UNIMI/R2OS1K/FQ45ZFdoi:10.13130/RD_UNIMI/R2OS1K/UQ1CUPdoi:10.13130/RD_UNIMI/R2OS1K/VIBEZZdoi:10.13130/RD_UNIMI/R2OS1K/62MUPMdoi:10.13130/RD_UNIMI/R2OS1K/HLSOQT
Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).
Pesaresi, Paolo(University of Milano)