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Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).","citation:dsDescriptionDate":"2020-01-03"},"subject":"Agricultural Sciences","citation:subtitle":"Sequences of the libraries to verify their quality","citation:depositor":"Pesaresi, Paolo","title":"Deliverable D2.1: Linear and cyclic peptide aptamer libraries","dateOfDeposit":"2020-01-03","@id":"https://doi.org/10.13130/RD_UNIMI/R2OS1K","@type":["ore:Aggregation","schema:Dataset"],"schema:version":"2.1","schema:name":"Deliverable D2.1: Linear and cyclic peptide aptamer libraries","schema:dateModified":"2024-01-30 15:41:24.737","schema:datePublished":"2020-01-04","dvcore:termsOfUse":"Restricted to the NoPEST partners.","dvcore:fileTermsOfAccess":{"dvcore:termsOfAccess":"Restricted to the members of NoPEST consortium","dvcore:fileRequestAccess":true},"schema:includedInDataCatalog":"UNIMI Dataverse","schema:isPartOf":{"schema:name":"FET-NoPEST","@id":"https://dataverse.unimi.it/dataverse/FET-NoPEST","schema:isPartOf":{"schema:name":"Dipartimento di Bioscienze","@id":"https://dataverse.unimi.it/dataverse/bioscienze","schema:isPartOf":{"schema:name":"UNIMI Dataverse","@id":"https://dataverse.unimi.it/dataverse/unimi","schema:description":"Unimi Dataverse is a repository for research data"}}},"ore:aggregates":[{"schema:description":"Description of the strategies used to generate the aptamer libraries","schema:name":"Deliverable_D2.1.pdf","dvcore:restricted":true,"schema:version":1,"dvcore:datasetVersionId":280,"@id":"doi:10.13130/RD_UNIMI/R2OS1K/FQ45ZF","schema:sameAs":"https://dataverse.unimi.it/api/access/datafile/:persistentId?persistentId=doi:10.13130/RD_UNIMI/R2OS1K/FQ45ZF","@type":"ore:AggregatedResource","schema:fileFormat":"application/pdf","dvcore:filesize":1450282,"dvcore:storageIdentifier":"s3://4s-dataverse-unimi:16f6b79b8db-ec900cfadb28","dvcore:rootDataFileId":-1,"dvcore:checksum":{"@type":"MD5","@value":"f2fadb8822b763a14baa82772224b417"}},{"schema:description":"200 sequences from linear and cyclic peptide libraries to test their quality","schema:name":"Sequences from generated Y2H peptide libraries.xlsx","dvcore:restricted":true,"schema:version":1,"dvcore:datasetVersionId":280,"@id":"doi:10.13130/RD_UNIMI/R2OS1K/HLSOQT","schema:sameAs":"https://dataverse.unimi.it/api/access/datafile/:persistentId?persistentId=doi:10.13130/RD_UNIMI/R2OS1K/HLSOQT&format=original","@type":"ore:AggregatedResource","schema:fileFormat":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","dvcore:filesize":22361,"dvcore:storageIdentifier":"s3://4s-dataverse-unimi:16fbec80d2a-31d6637e5df9","dvcore:currentIngestedName":"Sequences from generated Y2H peptide libraries.tab","dvcore:UNF":"UNF:6:fu9U/UKdlliZqAI94tsvXQ==","dvcore:rootDataFileId":-1,"dvcore:checksum":{"@type":"MD5","@value":"19fae289bd7ec91038f74270f959973e"}}],"schema:hasPart":["doi:10.13130/RD_UNIMI/R2OS1K/FQ45ZF","doi:10.13130/RD_UNIMI/R2OS1K/HLSOQT"]},"@context":{"author":"http://purl.org/dc/terms/creator","authorIdentifier":"http://purl.org/spar/datacite/AgentIdentifier","authorIdentifierScheme":"http://purl.org/spar/datacite/AgentIdentifierScheme","citation":"https://dataverse.org/schema/citation/","dateOfDeposit":"http://purl.org/dc/terms/dateSubmitted","dcterms":"http://purl.org/dc/terms/","dvcore":"https://dataverse.org/schema/core#","grantNumber":"https://schema.org/sponsor","ore":"http://www.openarchives.org/ore/terms/","schema":"http://schema.org/","subject":"http://purl.org/dc/terms/subject","title":"http://purl.org/dc/terms/title"}}