10.13130/RD_UNIMI/R2OS1KPesaresi, PaoloPaoloPesaresi0000-0002-3236-7005University of MilanoDeliverable D2.1: Linear and cyclic peptide aptamer librariesSequences of the libraries to verify their qualityUNIMI Dataverse2020Agricultural SciencesAptamerPesaresi, PaoloPaoloPesaresiUniversity of Milano2020-01-032024-01-3014502825375application/pdftext/tab-separated-values2.1Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs).European Commission828940