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Part 1: Document Description
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Citation |
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Title: |
16S rRNA gene sequencing data |
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Identification Number: |
doi:10.13130/RD_UNIMI/SFSEFZ |
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Distributor: |
Università degli Studi di Milano |
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Date of Distribution: |
2019-01-15 |
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Version: |
2 |
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Bibliographic Citation: |
Guglielmetti, Simone, 2019, "16S rRNA gene sequencing data", https://doi.org/10.13130/RD_UNIMI/SFSEFZ, Università degli Studi di Milano, V2 |
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Citation |
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Title: |
16S rRNA gene sequencing data |
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Identification Number: |
doi:10.13130/RD_UNIMI/SFSEFZ |
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Authoring Entity: |
Guglielmetti, Simone (DeFENS, Università degli Studi di Milano) |
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Distributor: |
Università degli Studi di Milano |
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Date of Distribution: |
2019-01-15 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences |
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Abstract: |
This dataset contains the following files:\r\n\r\n(1) 20 files \"fastq.gz\": files containing the raw sequencing data (FASTQ files) of the 16S rRNA gene profiling carried out on DNA extracted from BAL of antibiotic-treated (files from A1 to A5) and untreated mice (files from C1 to C5). The 16S rRNA gene was amplified using primers EUBF 5’-TCCTACGGGAGGCAGCAGT-3’ and EUBR 5’ -GGACTACCAGGGTATCTAATCCTGTT-3’ (DOI: 10.1099/00221287-148-1-257), which target the V3-V4 hypervariable regions, and sequenced using MiSeq Illumina® technology (2 x 300 paired-end MiSeq kit V3, set to encompass 467-bp amplicon) as previously described (DOI: 10.1371/journal.pone.0142334; DOI: 10.1111/trf.13477).\r\n\r\n(2) File named \"16S rRNA gene sequences.docx\": Word file containing the nucleotide sequences of part of the 16S rRNA gene amplified from the DNA of the bacterial strains isolated from the bronchoalveolar lavage fluid (BAL) of antibiotic-treated and untreated mice. Before each sequence, the first output of BLASTN search performed with default parameters against the \"16 ribosomal RNA sequences (Bacteria and Archaea)\" database.\r\n\r\nFor any additional need to this datasets, contact: simone.guglielmetti@unimi.it and lucia.sfondrini@unimi.it. |
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Methodology and Processing |
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16S rRNA gene sequencing data.docx |
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application/vnd.openxmlformats-officedocument.wordprocessingml.document |
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A1_R1.fastq.gz |
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application/x-gzip |
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A1_R2.fastq.gz |
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A2_R1.fastq.gz |
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A2_R2.fastq.gz |
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A3_R1.fastq.gz |
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A3_R2.fastq.gz |
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A4_R1.fastq.gz |
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A4_R2.fastq.gz |
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A5_R1.fastq.gz |
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A5_R2.fastq.gz |
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C1_R1.fastq.gz |
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C1_R2.fastq.gz |
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C2_R1.fastq.gz |
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C2_R2.fastq.gz |
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C3_R1.fastq.gz |
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C3_R2.fastq.gz |
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C4_R1.fastq.gz |
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C4_R2.fastq.gz |
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C5_R1.fastq.gz |
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application/x-gzip |
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C5_R2.fastq.gz |
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application/x-gzip |