Replication Data for 16S rRNA gene sequencing for: "Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases" (doi:10.13130/RD_UNIMI/SFSEFZ)

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Part 2: Study Description
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Document Description

Citation

Title:

Replication Data for 16S rRNA gene sequencing for: "Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases"

Identification Number:

doi:10.13130/RD_UNIMI/SFSEFZ

Distributor:

UNIMI Dataverse

Date of Distribution:

2019-01-15

Version:

2

Bibliographic Citation:

Guglielmetti, Simone, 2019, "Replication Data for 16S rRNA gene sequencing for: "Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases"", https://doi.org/10.13130/RD_UNIMI/SFSEFZ, UNIMI Dataverse, V2

Study Description

Citation

Title:

Replication Data for 16S rRNA gene sequencing for: "Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases"

Identification Number:

doi:10.13130/RD_UNIMI/SFSEFZ

Authoring Entity:

Guglielmetti, Simone (DeFENS, Università degli Studi di Milano)

Distributor:

UNIMI Dataverse

Access Authority:

Guglielmetti, Simone

Depositor:

Guglielmetti, Simone

Date of Deposit:

2018-07-23

Holdings Information:

https://doi.org/10.13130/RD_UNIMI/SFSEFZ

Study Scope

Keywords:

Medicine, Health and Life Sciences

Abstract:

This dataset contains the following files:\r\n\r\n(1) 20 files \"fastq.gz\": files containing the raw sequencing data (FASTQ files) of the 16S rRNA gene profiling carried out on DNA extracted from BAL of antibiotic-treated (files from A1 to A5) and untreated mice (files from C1 to C5). The 16S rRNA gene was amplified using primers EUBF 5’-TCCTACGGGAGGCAGCAGT-3’ and EUBR 5’ -GGACTACCAGGGTATCTAATCCTGTT-3’ (DOI: 10.1099/00221287-148-1-257), which target the V3-V4 hypervariable regions, and sequenced using MiSeq Illumina® technology (2 x 300 paired-end MiSeq kit V3, set to encompass 467-bp amplicon) as previously described (DOI: 10.1371/journal.pone.0142334; DOI: 10.1111/trf.13477).\r\n\r\n(2) File named \"16S rRNA gene sequences.docx\": Word file containing the nucleotide sequences of part of the 16S rRNA gene amplified from the DNA of the bacterial strains isolated from the bronchoalveolar lavage fluid (BAL) of antibiotic-treated and untreated mice. Before each sequence, the first output of BLASTN search performed with default parameters against the \"16 ribosomal RNA sequences (Bacteria and Archaea)\" database.\r\n\r\nFor any additional need to this datasets, contact: simone.guglielmetti@unimib.it and lucia.sfondrini@unimi.it.

Methodology and Processing

Sources Statement

Data Access

Other Study Description Materials

Related Publications

Citation

Identification Number:

https://doi.org/10.1016/j.celrep.2018.08.090

Bibliographic Citation:

Le Noci V, Guglielmetti S, Arioli S, Camisaschi C, Bianchi F, Sommariva M, Storti C, Triulzi T, Castelli C, Balsari A, Tagliabue E, Sfondrini L. Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases. Cell Rep. 2018 Sep 25;24(13):3528-3538. doi: 10.1016/j.celrep.2018.08.090. PMID: 30257213.

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16S rRNA gene sequencing data.docx

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application/vnd.openxmlformats-officedocument.wordprocessingml.document

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A1_R1.fastq.gz

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application/x-gzip

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A1_R2.fastq.gz

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A2_R1.fastq.gz

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A2_R2.fastq.gz

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A3_R1.fastq.gz

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A3_R2.fastq.gz

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A4_R1.fastq.gz

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A4_R2.fastq.gz

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A5_R1.fastq.gz

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A5_R2.fastq.gz

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C1_R1.fastq.gz

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C1_R2.fastq.gz

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C2_R1.fastq.gz

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C2_R2.fastq.gz

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C3_R1.fastq.gz

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C3_R2.fastq.gz

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C4_R1.fastq.gz

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C4_R2.fastq.gz

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application/x-gzip

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C5_R1.fastq.gz

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application/x-gzip

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C5_R2.fastq.gz

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application/x-gzip