Replication Data for Proteomic screening identifies megakaryocyte-derived PF4/Cxcl4 as a critical driver of myelofibrosis (doi:10.13130/RD_UNIMI/RI3IDJ)

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Part 1: Document Description
Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Part 5: Other Study-Related Materials
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Document Description

Citation

Title:

Replication Data for Proteomic screening identifies megakaryocyte-derived PF4/Cxcl4 as a critical driver of myelofibrosis

Identification Number:

doi:10.13130/RD_UNIMI/RI3IDJ

Distributor:

UNIMI Dataverse

Date of Distribution:

2023-05-30

Version:

2

Bibliographic Citation:

Capitanio, Daniele, 2023, "Replication Data for Proteomic screening identifies megakaryocyte-derived PF4/Cxcl4 as a critical driver of myelofibrosis", https://doi.org/10.13130/RD_UNIMI/RI3IDJ, UNIMI Dataverse, V2, UNF:6:EIynqsgx5iLtwL7IRdTYTg== [fileUNF]

Study Description

Citation

Title:

Replication Data for Proteomic screening identifies megakaryocyte-derived PF4/Cxcl4 as a critical driver of myelofibrosis

Identification Number:

doi:10.13130/RD_UNIMI/RI3IDJ

Authoring Entity:

Capitanio, Daniele (University of Milan)

Distributor:

UNIMI Dataverse

Access Authority:

Capitanio, Daniele

Depositor:

Capitanio, Daniele

Date of Deposit:

2023-05-17

Holdings Information:

https://doi.org/10.13130/RD_UNIMI/RI3IDJ

Study Scope

Keywords:

Medicine, Health and Life Sciences

Abstract:

This dataverse include the LC-MS/MS proteomics datasets of changed proteins generated in the study: "Proteomic screening identifies megakaryocyte-derived PF4/Cxcl4 as a critical driver of myelofibrosis" by Daniele Capitanio, Francesca R. Calledda, Vittorio Abbonante, Daniele Cattaneo, Manuela Moriggi, Niccolò Bartalucci, Cristina Bucelli, Delfina Tosi, Umberto Gianelli, Alessandro Maria Vannucchi, Alessandra Iurlo, Cecilia Gelfi, Alessandra Balduini, and Alessandro Malara. Data were generated by LC-ESI-MS/MS with label-free quantitation. Mass spectra were analyzed using MaxQuant software (Max-Planck-Institute of Biochemistry, Munich, Germany, version 1.6.17.0). Statistical analyses were performed using the Perseus software (Max Planck Institute of Biochemistry, Munich, Germany, version 1.6.15.0). For statistical analysis, Student's t-test with a p-value threshold of 0.05 was applied. To exclude the presence of false positives from the analysis, Benjamini–Hochberg false discovery rate test was applied.

Methodology and Processing

Sources Statement

Data Access

Other Study Description Materials

Related Publications

Citation

Identification Number:

https://doi.org/10.1182/blood-2023-188967

Bibliographic Citation:

Alessandro Malara, Daniele Capitanio, Francesca Rossella Calledda, Vittorio Abbonante, Daniele Cattaneo, Alessandra Iurlo, Cecilia Gelfi, Alessandra Balduini; "Proteomic Screening Identifies Megakaryocyte Derived PF4/Cxcl4 As a Critical Driver of Myelofibrosis". Blood 2023; 142 (Supplement 1)

File Description--f137827

File: Bonemarrow proteome.tab

  • Number of cases: 152

  • No. of variables per record: 24

  • Type of File: text/tab-separated-values

Notes:

UNF:6:kQIHg8sj3vOOhwmvc/OA9w==

File Description--f137828

File: Megakaryocytes proteome.tab

  • Number of cases: 51

  • No. of variables per record: 24

  • Type of File: text/tab-separated-values

Notes:

UNF:6:oVj2BjsXpYKZYRJh7tamPg==

File Description--f137829

File: Platelets proteome.tab

  • Number of cases: 110

  • No. of variables per record: 24

  • Type of File: text/tab-separated-values

Notes:

UNF:6:AzDH2vPtbMS6ITO2D9yutA==

Variable Description

List of Variables:

Variables

LFQintensityControl-01

f137827 Location:

Summary Statistics: Mean 23.432580263157895; StDev 2.0668055706875523; Valid 152.0; Max. 32.4258; Min. 18.4124;

Variable Format: numeric

Notes: UNF:6:oY8L1t/H/4snJTVPa7wNfA==

LFQintensityControl-02

f137827 Location:

Summary Statistics: Valid 152.0; StDev 2.0751209233650036; Min. 18.4264; Mean 23.40931907894737; Max. 32.4786;

Variable Format: numeric

Notes: UNF:6:g8NyZCxru8fb93MPrttkSA==

LFQintensityTPOhigh-01

f137827 Location:

Summary Statistics: Min. 19.6385; Mean 23.544623684210528; Max. 31.2624; StDev 2.165789139991598; Valid 152.0;

Variable Format: numeric

Notes: UNF:6:13bAMQ9bO03OGJtaQf5UVA==

LFQintensityTPOhigh-02

f137827 Location:

Summary Statistics: Valid 152.0; Mean 23.551380263157895; Max. 31.2318; Min. 19.5621; StDev 2.167362024330162;

Variable Format: numeric

Notes: UNF:6:e2cqQfod/zAPJIZ1LSXkpg==

C:Student'sT-testSignificant

f137827 Location:

Variable Format: character

Notes: UNF:6:QaSPTg6pbA/zqaJGNny4Mw==

N:Peptides

f137827 Location:

Summary Statistics: Max. 56.0; StDev 9.030926975430171; Valid 152.0; Min. 2.0; Mean 9.677631578947373;

Variable Format: numeric

Notes: UNF:6:7VGBVAr3vSRMk8uJQNKiqw==

N:Razor+uniquepeptides

f137827 Location:

Summary Statistics: Max. 56.0; StDev 8.842999034688448; Valid 152.0; Min. 2.0; Mean 9.243421052631573

Variable Format: numeric

Notes: UNF:6:/4YF5SbMA915X67wGjgtiQ==

N:Uniquepeptides

f137827 Location:

Summary Statistics: Mean 8.2171052631579; Max. 56.0; Valid 152.0; Min. 0.0; StDev 8.39024801562389;

Variable Format: numeric

Notes: UNF:6:ecxVley+DW2qwqxtTZuZxQ==

N:Sequencecoverage[%]

f137827 Location:

Summary Statistics: Max. 95.2; Mean 31.492763157894736; Valid 152.0; Min. 2.6; StDev 16.569021209400816

Variable Format: numeric

Notes: UNF:6:sjDrbseSqisKUpxBTb8Tcw==

N:Unique+razorsequencecoverage[%]

f137827 Location:

Summary Statistics: Mean 30.656578947368423; Min. 1.9; Valid 152.0; Max. 95.2; StDev 16.824704600902606

Variable Format: numeric

Notes: UNF:6:Tp8pMAhIVIg1/B//Fu4aLA==

N:Uniquesequencecoverage[%]

f137827 Location:

Summary Statistics: Valid 152.0; StDev 15.74709861960347; Max. 77.5; Mean 27.74671052631579; Min. 0.0

Variable Format: numeric

Notes: UNF:6:EGjnt5HOVpJIwrrWfjh/wA==

N:Mol.weight[kDa]

f137827 Location:

Summary Statistics: Mean 53.26435723684211; Max. 274.63; Min. 6.4374; Valid 152.0; StDev 52.0167395288071

Variable Format: numeric

Notes: UNF:6:M8xTZTpp5mxpOfpfIJmCsg==

N:Q-value

f137827 Location:

Summary Statistics: StDev 0.0; Max. 0.0; Min. 0.0; Mean 0.0; Valid 152.0

Variable Format: numeric

Notes: UNF:6:wvz02l5hZ6jMwGo7f7dqeA==

N:Score

f137827 Location:

Summary Statistics: Max. 323.31; StDev 86.42919770967181; Mean 88.90986842105264; Valid 152.0; Min. 12.62;

Variable Format: numeric

Notes: UNF:6:zUaVIrRu3zqFVbJONCUp7g==

N:Intensity

f137827 Location:

Summary Statistics: Valid 152.0; Mean 3.925871677631578E8; StDev 1.9331345995700874E9; Max. 2.0149E10; Min. 6049700.0

Variable Format: numeric

Notes: UNF:6:TlGDldUw4K9XkqCfG33TUA==

N:MS/MScount

f137827 Location:

Summary Statistics: Max. 453.0; Valid 152.0; StDev 66.721719890853; Min. 8.0; Mean 50.01315789473684

Variable Format: numeric

Notes: UNF:6:fRZATRuMBPZEMymssDhXDQ==

N:Student'sT-testp-value

f137827 Location:

Summary Statistics: Min. 3.67924E-6; Valid 152.0; StDev 0.00796322012509661; Mean 0.007745272097631579; Max. 0.0302011

Variable Format: numeric

Notes: UNF:6:34bX8F7Ebgi9f3Y+RwWxRg==

N:Student'sT-testq-value

f137827 Location:

Summary Statistics: Min. 8.97734E-4; Valid 152.0; Max. 0.0488018; StDev 0.01200102071720299; Mean 0.018270529763157895

Variable Format: numeric

Notes: UNF:6:RAbwtom/IRs+61c2bJxLkQ==

N:Student'sT-testDifference(Logfoldchange)TPOhigh/Control

f137827 Location:

Summary Statistics: Valid 152.0; Mean 0.1270482677631581; StDev 1.0510042308758236; Max. 4.07449; Min. -4.29254

Variable Format: numeric

Notes: UNF:6:zZEp2mGdwdfpLu+FC00lVQ==

N:Student'sT-testTeststatistic

f137827 Location:

Summary Statistics: Min. -521.339; Mean -9.901448618421064; Max. 115.601; StDev 62.60096808324173; Valid 152.0;

Variable Format: numeric

Notes: UNF:6:iFS7ItyILoqqHBeehcFZug==

T:ProteinIDs

f137827 Location:

Variable Format: character

Notes: UNF:6:AhMQ8PaCtWlSpqq1T0CRng==

T:MajorityproteinIDs

f137827 Location:

Variable Format: character

Notes: UNF:6:5JX8aWwhmBSY0ZJIaxgH1g==

T:Proteinnames

f137827 Location:

Variable Format: character

Notes: UNF:6:v2UE/Qgcw5sIaNg/oMrmfA==

T:Genenames

f137827 Location:

Variable Format: character

Notes: UNF:6:ltzKPh4nepdjrklVi554kA==

LFQintensityControl-1

f137828 Location:

Summary Statistics: Min. 20.2442; Valid 51.0; Max. 27.6045; Mean 23.239156862745098; StDev 1.9909598379932127

Variable Format: numeric

Notes: UNF:6:b/COsxkC4pvRKO7dOCaDaQ==

LFQintensityControl-2

f137828 Location:

Summary Statistics: Max. 27.4618; Valid 51.0; Min. 19.9241; Mean 23.289649019607843; StDev 2.000979408926794;

Variable Format: numeric

Notes: UNF:6:0XY8ZOiMy9OvG1BiIREV+A==

LFQintensityTPOhigh-1

f137828 Location:

Summary Statistics: Max. 30.2773; StDev 1.8627925116203952; Min. 22.6111; Mean 25.93438431372549; Valid 51.0

Variable Format: numeric

Notes: UNF:6:vGSZOYTpvDbyWBm0tnSFNA==

LFQintensityTPOhigh-2

f137828 Location:

Summary Statistics: StDev 1.8686585325832215; Mean 25.91623137254902; Min. 22.6231; Valid 51.0; Max. 30.3685

Variable Format: numeric

Notes: UNF:6:1aBfKp/TF8lXHN/iomVLzw==

C:Student'sT-testSignificant

f137828 Location:

Variable Format: character

Notes: UNF:6:DcTIqyEBu1ZeBmh0wOK4bQ==

N:Peptides

f137828 Location:

Summary Statistics: Valid 51.0; Mean 17.549019607843135; Max. 89.0; StDev 18.089017359149384; Min. 3.0

Variable Format: numeric

Notes: UNF:6:UEuk86TAJpCfnZ36IiuSxw==

N:Razor+uniquepeptides

f137828 Location:

Summary Statistics: Valid 51.0; Mean 17.098039215686278; StDev 18.221146947391414; Min. 3.0; Max. 89.0

Variable Format: numeric

Notes: UNF:6:JEcOhvbtaMTSczndxlyJWQ==

N:Uniquepeptides

f137828 Location:

Summary Statistics: Valid 51.0; Min. 1.0; StDev 18.237732917245616; Mean 14.49019607843137; Max. 89.0

Variable Format: numeric

Notes: UNF:6:IDpZRqDIJzfQwG/m9n1pyQ==

N:Sequencecoverage[%]

f137828 Location:

Summary Statistics: Mean 43.825490196078434; Valid 51.0; StDev 15.1368403986731; Max. 84.4; Min. 1.1

Variable Format: numeric

Notes: UNF:6:qlJ3JpTKtl1VbujDITJsDA==

N:Unique+razorsequencecoverage[%]

f137828 Location:

Summary Statistics: Valid 51.0; Min. 0.7; Max. 77.6; Mean 42.845098039215685; StDev 15.282817982629906;

Variable Format: numeric

Notes: UNF:6:MDzAY6JcO5Gwo91mojH+rw==

N:Uniquesequencecoverage[%]

f137828 Location:

Summary Statistics: Min. 0.7; Valid 51.0; Max. 71.9; Mean 36.0843137254902; StDev 17.078868493539257

Variable Format: numeric

Notes: UNF:6:vkfQjIfDOSUFVeHJMPIddw==

N:Mol.weight[kDa]

f137828 Location:

Summary Statistics: Max. 503.9; Min. 10.294; StDev 85.64942520460035; Valid 51.0; Mean 62.72862745098039;

Variable Format: numeric

Notes: UNF:6:uPC8NJvIjhbsLGeCwkY1HQ==

N:Q-value

f137828 Location:

Summary Statistics: Mean 0.0; Max. 0.0; Valid 51.0; Min. 0.0; StDev 0.0

Variable Format: numeric

Notes: UNF:6:6gPOQx1+zbo7w5YK07N2uQ==

N:Score

f137828 Location:

Summary Statistics: Min. 21.722; Max. 323.31; Mean 133.04070588235294; StDev 84.91451209452813; Valid 51.0

Variable Format: numeric

Notes: UNF:6:3CMYAWmY8R/xOq2j36ksFQ==

N:Intensity

f137828 Location:

Summary Statistics: StDev 1.3278674027845623E9; Mean 7.7629068627451E8; Min. 3.7879E7; Valid 51.0; Max. 6.3293E9

Variable Format: numeric

Notes: UNF:6:0FvLpHFG+ZTFIC2c2LmX1g==

N:MS/MScount

f137828 Location:

Summary Statistics: Valid 51.0; StDev 62.10517328709105; Max. 288.0; Min. 12.0; Mean 74.54901960784314;

Variable Format: numeric

Notes: UNF:6:w8DmdsvClTjqGUWDYSkv4w==

N:Student'sT-testp-value

f137828 Location:

Summary Statistics: StDev 0.005017853070107859; Min. 1.40838E-5; Max. 0.0213326; Valid 51.0; Mean 0.003662720547058823

Variable Format: numeric

Notes: UNF:6:3zk7kmkRkELyPkDtDvXUCg==

N:Student'sT-testq-value

f137828 Location:

Summary Statistics: Mean 0.00674321356862745; StDev 0.007183070553843874; Valid 51.0; Max. 0.0305349; Min. 5.18834E-4

Variable Format: numeric

Notes: UNF:6:/StspCFF1fR3Pkk8kxmAZA==

N:Student'sT-testDifference(Logfoldchange)TPOhigh/Control

f137828 Location:

Summary Statistics: Valid 51.0; StDev 1.2084265716175286; Mean 2.6609064705882353; Max. 5.37458; Min. -1.03928;

Variable Format: numeric

Notes: UNF:6:0xv5STsA3DiA7J8OnrBO6g==

N:Student'sT-testTeststatistic

f137828 Location:

Summary Statistics: Min. -266.462; StDev 58.39947869700366; Valid 51.0; Max. 8.37715; Mean -48.89189921568627

Variable Format: numeric

Notes: UNF:6:cuySGOIrK3cYlHZnADlUtg==

T:ProteinIDs

f137828 Location:

Variable Format: character

Notes: UNF:6:WFDunEs21pD30c0rP1MQBg==

T:MajorityproteinIDs

f137828 Location:

Variable Format: character

Notes: UNF:6:xzsRUdgc9gw7hZtaa2TRXQ==

T:Proteinnames

f137828 Location:

Variable Format: character

Notes: UNF:6:2KNewRfJhNvFqa2QI1n9yg==

T:Genenames

f137828 Location:

Variable Format: character

Notes: UNF:6:eWrNx8Od/sny58P+SLFQVA==

LFQintensityControl-1

f137829 Location:

Summary Statistics: Min. 20.2771; Mean 24.140583636363637; Valid 110.0; Max. 32.6758; StDev 2.413111051540982;

Variable Format: numeric

Notes: UNF:6:EFvPHk+iDD2af63AgCW+sg==

LFQintensityControl-2

f137829 Location:

Summary Statistics: Valid 110.0; Mean 24.145446363636363; StDev 2.409192278808617; Min. 20.4012; Max. 32.6569

Variable Format: numeric

Notes: UNF:6:/y9jwNc6YzrWi9C5PlO7VA==

LFQintensityTPOhigh-1

f137829 Location:

Summary Statistics: Valid 110.0; StDev 2.233079535364274; Min. 20.2486; Max. 32.0712; Mean 24.216641818181817;

Variable Format: numeric

Notes: UNF:6:0eIt8aRYfPu1z82f5GJGtQ==

LFQintensityTPOhigh-2

f137829 Location:

Summary Statistics: Max. 32.0786; Mean 24.209636363636363; StDev 2.2348447759954144; Min. 20.2383; Valid 110.0;

Variable Format: numeric

Notes: UNF:6:L0YLCKdGFmwkyETjyVNBkA==

C:Student'sT-testSignificant

f137829 Location:

Variable Format: character

Notes: UNF:6:TSiBARAe1MOzLfdaJmiyuw==

N:Peptides

f137829 Location:

Summary Statistics: Mean 11.018181818181818; StDev 11.339612971876944; Min. 2.0; Max. 88.0; Valid 110.0

Variable Format: numeric

Notes: UNF:6:Zfy7ka/nNYcq2OwMOZj5Fg==

N:Razor+uniquepeptides

f137829 Location:

Summary Statistics: StDev 11.415132692117464; Min. 2.0; Mean 10.545454545454545; Max. 88.0; Valid 110.0

Variable Format: numeric

Notes: UNF:6:vKAHqA4L0dNPuIJMrn4pwA==

N:Uniquepeptides

f137829 Location:

Summary Statistics: Min. 0.0; StDev 11.501042488194829; Valid 110.0; Mean 9.31818181818182; Max. 88.0;

Variable Format: numeric

Notes: UNF:6:EMeTmklXGd5Xxiz2gFAfIA==

N:Sequencecoverage[%]

f137829 Location:

Summary Statistics: Valid 110.0; Max. 95.2; Mean 28.173636363636362; Min. 3.6; StDev 16.32200449369634

Variable Format: numeric

Notes: UNF:6:+WMdkWY5Seq4AuQDUxZdSg==

N:Unique+razorsequencecoverage[%]

f137829 Location:

Summary Statistics: Mean 27.01090909090909; Max. 95.2; Min. 1.0; StDev 16.599150776633756; Valid 110.0

Variable Format: numeric

Notes: UNF:6:V4ypvyXw00QcweYuCz57MA==

N:Uniquesequencecoverage[%]

f137829 Location:

Summary Statistics: StDev 15.23101125729783; Min. 0.0; Mean 23.115454545454547; Valid 110.0; Max. 77.5

Variable Format: numeric

Notes: UNF:6:wtwFeJd6dI9DSVGBWJ/WAw==

N:Mol.weight[kDa]

f137829 Location:

Summary Statistics: Min. 8.7279; Max. 280.5; Mean 59.88496272727273; StDev 53.52048232932722; Valid 110.0;

Variable Format: numeric

Notes: UNF:6:GtM4HRUB7ZaVTlL4Se2xqA==

N:Q-value

f137829 Location:

Summary Statistics: Min. 0.0; StDev 0.0; Mean 0.0; Valid 110.0; Max. 0.0;

Variable Format: numeric

Notes: UNF:6:2gonOxP1ek6ET/pNQBkwfA==

N:Score

f137829 Location:

Summary Statistics: Min. 12.697; StDev 89.56540627325467; Valid 110.0; Max. 323.31; Mean 98.04126363636364

Variable Format: numeric

Notes: UNF:6:TslWVAl32h5ZwDUrgc8jWA==

N:Intensity

f137829 Location:

Summary Statistics: Valid 110.0; Mean 6.990227309090904E8; Max. 2.9231E10; StDev 3.0657456296466393E9; Min. 9187500.0

Variable Format: numeric

Notes: UNF:6:dxsIQqfpSF/TMb1Iv0wuEw==

N:MS/MScount

f137829 Location:

Summary Statistics: Mean 56.4; Valid 110.0; Max. 484.0; StDev 79.05023370959307; Min. 7.0;

Variable Format: numeric

Notes: UNF:6:xe4btiCN2nfTuw6lYdf5JQ==

N:Student'sT-testp-value

f137829 Location:

Summary Statistics: StDev 0.008456953551748029; Mean 0.007239718635181819; Valid 110.0; Min. 4.93537E-6; Max. 0.0297767

Variable Format: numeric

Notes: UNF:6:bFyr4rL1spftvohzEzidVg==

N:Student'sT-testq-value

f137829 Location:

Summary Statistics: StDev 0.013698154089534115; Mean 0.01644275779090909; Max. 0.0492669; Min. 8.98237E-4; Valid 110.0

Variable Format: numeric

Notes: UNF:6:q5Q5vJE57jwqxQ2d+dRg7Q==

N:Student'sT-testDifference(Logfoldchange)TPOhigh/Control

f137829 Location:

Summary Statistics: Max. 1.27104; Valid 110.0; StDev 0.7984017989065092; Min. -2.81419; Mean 0.0701211963636366

Variable Format: numeric

Notes: UNF:6:FrGmfqG8jMljLJQTd+k6Ig==

N:Student'sT-testTeststatistic

f137829 Location:

Summary Statistics: StDev 59.65327276387067; Mean -2.8945563636363705; Max. 450.131; Valid 110.0; Min. -155.519

Variable Format: numeric

Notes: UNF:6:mMftOczRRpJGjZsEg5CUlw==

T:ProteinIDs

f137829 Location:

Variable Format: character

Notes: UNF:6:WBti8bjbCfqwJNsM6MJPug==

T:MajorityproteinIDs

f137829 Location:

Variable Format: character

Notes: UNF:6:iTkGwYacXqdpBnjPXnUb9A==

T:Proteinnames

f137829 Location:

Variable Format: character

Notes: UNF:6:kjvP2sTkzPTU/CPJP7bE+Q==

T:Genenames

f137829 Location:

Variable Format: character

Notes: UNF:6:BtkPEevHTW9/mqJ6KYnb5g==

Other Study-Related Materials

Label:

Read me file.pdf

Notes:

application/pdf