Sitia L. et al. Cells, 2021, doi:10.3390/cells10020328 (ICPSR doi:10.13130/RD_UNIMI/SKRLH0)

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Part 1: Document Description
Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Entire Codebook

Document Description

Citation

Title:

Sitia L. et al. Cells, 2021, doi:10.3390/cells10020328

Identification Number:

doi:10.13130/RD_UNIMI/SKRLH0

Distributor:

UNIMI Dataverse

Date of Distribution:

2021-02-15

Version:

1

Bibliographic Citation:

Mazzucchelli, Serena, 2021, "Sitia L. et al. Cells, 2021, doi:10.3390/cells10020328", https://doi.org/10.13130/RD_UNIMI/SKRLH0, UNIMI Dataverse, V1, UNF:6:8zE4cXNoQVCZc+paF4XK3A== [fileUNF]

Study Description

Citation

Title:

Sitia L. et al. Cells, 2021, doi:10.3390/cells10020328

Identification Number:

doi:10.13130/RD_UNIMI/SKRLH0

Authoring Entity:

Mazzucchelli, Serena (University of Milan)

Distributor:

UNIMI Dataverse

Access Authority:

Mazzucchelli, Serena

Depositor:

Mazzucchelli, Serena

Date of Deposit:

2021-02-03

Study Scope

Keywords:

Medicine, Health and Life Sciences

Abstract:

This dataset contains raw data from Sitia, L.; Bonizzi, A.; Mazzucchelli, S.; Negri, S.; Sottani, C.; Grignani, E.; Rizzuto, M.A.; Prosperi, D.; Sorren-tino, L.; Morasso, C.; et al. Selective targeting of Cancer-Associated Fi-broblasts by engineered H-ferritin nanocages loaded with navitoclax. Cells 2021, 10.3390/cells10020328

Methodology and Processing

Sources Statement

Data Access

Notes:

CC0 Waiver

Other Study Description Materials

Related Publications

Citation

Bibliographic Citation:

Sitia, L.; Bonizzi, A.; Mazzucchelli, S.; Negri, S.; Sottani, C.; Grignani, E.; Rizzuto, M.A.; Prosperi, D.; Sorren-tino, L.; Morasso, C.; et al. Selective targeting of Cancer-Associated Fi-broblasts by engineered H-ferritin nanocages loaded with navitoclax. Cells 2021, doi:10.3390/cells10020328

File Description--f5968

File: Binding with HFn-FAP.tab

  • Number of cases: 6

  • No. of variables per record: 7

  • Type of File: text/tab-separated-values

Notes:

UNF:6:F0XTs/fNeIcYC7XjfnVIpQ==

File Description--f5971

File: Biodistribution data.tab

  • Number of cases: 5

  • No. of variables per record: 5

  • Type of File: text/tab-separated-values

Notes:

UNF:6:H5sRVSomBoSpQtxVMkx3Hg==

File Description--f5965

File: FAP and TfR1 expression.tab

  • Number of cases: 9

  • No. of variables per record: 7

  • Type of File: text/tab-separated-values

Notes:

UNF:6:ygdt6NwR+HoL1m2n7YcLyg==

File Description--f5970

File: HNav activity and stability.tab

  • Number of cases: 112

  • No. of variables per record: 5

  • Type of File: text/tab-separated-values

Notes:

UNF:6:cv7+vVLJuRGNT+SghR4BCA==

File Description--f5972

File: HNAV and HNav-FAP loading and recoveries.tab

  • Number of cases: 999

  • No. of variables per record: 26

  • Type of File: text/tab-separated-values

Notes:

UNF:6:bijeiGLwnfaZc8jJMdF8mQ==

File Description--f5967

File: MTS Control HFn HFAP and CuSO4 .tab

  • Number of cases: 7

  • No. of variables per record: 6

  • Type of File: text/tab-separated-values

Notes:

UNF:6:bvqx1JZqvc3QQW0e0+va1Q==

File Description--f5969

File: MTS Nav HNav and HNav-FAP activity.tab

  • Number of cases: 6

  • No. of variables per record: 20

  • Type of File: text/tab-separated-values

Notes:

UNF:6:kc1d+G6/FerIyPl7AEvUlA==

File Description--f5966

File: Nav uptake .tab

  • Number of cases: 13

  • No. of variables per record: 9

  • Type of File: text/tab-separated-values

Notes:

UNF:6:5aPQ+FnDVFw9UBZOqJCKHQ==

Variable Description

List of Variables:

Variables

ContributionofHFn-FAPinbindingFAP+(CAFsandHMfs)versusFAP-(MDA-MB-231)cells

f5968 Location:

Variable Format: character

Notes: UNF:6:uj1cQ+IMQgl9wmrYoV0HpQ==

B

f5968 Location:

Variable Format: character

Notes: UNF:6:476BZY2MDXn5alA73fWUOA==

C

f5968 Location:

Variable Format: character

Notes: UNF:6:uucm8prZfoj89zdqMxTCTA==

D

f5968 Location:

Variable Format: character

Notes: UNF:6:1VWQknjvfFilu6Xd/WAvrA==

E

f5968 Location:

Variable Format: character

Notes: UNF:6:Io6ONHl3B42O9hXHt8fk7Q==

F

f5968 Location:

Variable Format: character

Notes: UNF:6:EIf5k45/qmuK1/oLlBPuzA==

G

f5968 Location:

Variable Format: character

Notes: UNF:6:jCZk+ZQz2DRFW+y74jlLzg==

Quantificationofthefluorescentsignalmeasuredinthetumorsbyexvivoimaging,expressedasaverageradiantefficiency

f5971 Location:

Variable Format: character

Notes: UNF:6:cmRMQ/KTq4bihY6utIWSnw==

B

f5971 Location:

Variable Format: character

Notes: UNF:6:Z6bwLle5wSYA6nR0AI+8dg==

C

f5971 Location:

Variable Format: character

Notes: UNF:6:OOgg4gRixlqz3c6lqOhzPg==

D

f5971 Location:

Variable Format: character

Notes: UNF:6:p4+YGA9I0Hs98+32q/8RuQ==

E

f5971 Location:

Variable Format: character

Notes: UNF:6:XlHtFDT5qaH0Wr2e15H5cQ==

Fapexpression

f5965 Location:

Variable Format: character

Notes: UNF:6:e865OMVZTbg+kH5dTKR1SQ==

CAF

f5965 Location:

Summary Statistics: StDev 1.6350025484179915; Max. 62.54; Valid 3.0; Mean 60.903333333333336; Min. 59.27

Variable Format: numeric

Notes: UNF:6:yOH2o7xTwMnR2AkMaYLioQ==

4T1

f5965 Location:

Summary Statistics: Valid 3.0; Max. 1.97; Mean 1.1233333333333333; Min. 0.28; StDev 0.8450049309520823

Variable Format: numeric

Notes: UNF:6:b2gj9qZI8iLpboLwUz83tQ==

HMf

f5965 Location:

Summary Statistics: Max. 98.23; Min. 50.62; Valid 9.0; Mean 78.87; StDev 20.221194326745394

Variable Format: numeric

Notes: UNF:6:/+Z5o/ucVcPvRmgrigIhIw==

MDA-MB-231

f5965 Location:

Summary Statistics: Mean 0.305; Max. 0.7; Min. 0.07; StDev 0.3011644069275119; Valid 4.0;

Variable Format: numeric

Notes: UNF:6:YdK90e00Y6GvJdfVCF1o2g==

F

f5965 Location:

Summary Statistics: Valid 0.0; Min. NaN; StDev NaN; Mean NaN; Max. NaN;

Variable Format: numeric

Notes: UNF:6:6kmqn29s8tU9RU5ii6WjOQ==

G

f5965 Location:

Summary Statistics: Max. NaN; StDev NaN; Min. NaN; Mean NaN; Valid 0.0

Variable Format: numeric

Notes: UNF:6:6kmqn29s8tU9RU5ii6WjOQ==

sample

f5970 Location:

Variable Format: character

Notes: UNF:6:XcA8E0lCkptYsOsM0qQV4Q==

Image

f5970 Location:

Variable Format: character

Notes: UNF:6:LEPSXjVKoXH1Wz0YoHzquA==

totalcells

f5970 Location:

Summary Statistics: Max. 199.0; Valid 112.0; Mean 45.23214285714286; StDev 26.26064165003018; Min. 12.0

Variable Format: numeric

Notes: UNF:6:SW3RXntlOrrk7W2/7V8RTw==

apoptoticnuclei

f5970 Location:

Summary Statistics: Valid 112.0; Max. 13.0; Mean 2.1875; Min. 0.0; StDev 2.9209749390562383

Variable Format: numeric

Notes: UNF:6:ZsLrcDa2/wDx7nmRKXnzrg==

%apoptoticnuclei

f5970 Location:

Summary Statistics: Min. 0.0; Valid 112.0; Mean 5.467761553980776; Max. 34.375; StDev 7.327715831610583

Variable Format: numeric

Notes: UNF:6:ydlxrTib0jGBSaxdyX+CqA==

A

f5972 Location:

Variable Format: character

Notes: UNF:6:m2qyp8Y6Eikz9gFc078R1g==

B

f5972 Location:

Variable Format: character

Notes: UNF:6:j369Q9Q6Lf+kToc9Xg4Erw==

C

f5972 Location:

Variable Format: character

Notes: UNF:6:FphAXsR2vMxQtuyvXCzizQ==

D

f5972 Location:

Variable Format: character

Notes: UNF:6:fQJok/IEV8Eg9FYiu2UK2Q==

E

f5972 Location:

Variable Format: character

Notes: UNF:6:rTd/cY1N1dQzw7uZQtrbig==

F

f5972 Location:

Variable Format: character

Notes: UNF:6:u6m1zSJXVZo2LV9Et61SXw==

G

f5972 Location:

Variable Format: character

Notes: UNF:6:S5opGAGLHxtm4ne1WqlSiQ==

H

f5972 Location:

Variable Format: character

Notes: UNF:6:0Waspe0sflzn5Nk6tAgDBQ==

I

f5972 Location:

Variable Format: character

Notes: UNF:6:dsn8MHl8vgJ6dO4/EjKbDw==

J

f5972 Location:

Summary Statistics: StDev NaN; Valid 0.0; Min. NaN; Max. NaN; Mean NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

K

f5972 Location:

Summary Statistics: Min. NaN; Max. NaN; Mean NaN; StDev NaN; Valid 0.0;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

L

f5972 Location:

Summary Statistics: Mean NaN; Max. NaN; Min. NaN; StDev NaN; Valid 0.0

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

M

f5972 Location:

Summary Statistics: Max. NaN; Mean NaN; Valid 0.0; Min. NaN; StDev NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

N

f5972 Location:

Summary Statistics: Mean NaN; Min. NaN; Valid 0.0; Max. NaN; StDev NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

O

f5972 Location:

Summary Statistics: Valid 0.0; Min. NaN; Max. NaN; Mean NaN; StDev NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

P

f5972 Location:

Summary Statistics: Valid 0.0; Mean NaN; Max. NaN; StDev NaN; Min. NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

Q

f5972 Location:

Summary Statistics: StDev NaN; Mean NaN; Valid 0.0; Max. NaN; Min. NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

R

f5972 Location:

Summary Statistics: Min. NaN; Max. NaN; Mean NaN; Valid 0.0; StDev NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

S

f5972 Location:

Summary Statistics: Min. NaN; Valid 0.0; Mean NaN; StDev NaN; Max. NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

T

f5972 Location:

Summary Statistics: StDev NaN; Valid 0.0; Mean NaN; Min. NaN; Max. NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

U

f5972 Location:

Summary Statistics: StDev NaN; Mean NaN; Min. NaN; Valid 0.0; Max. NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

V

f5972 Location:

Summary Statistics: Mean NaN; Max. NaN; Min. NaN; StDev NaN; Valid 0.0;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

W

f5972 Location:

Summary Statistics: Max. NaN; Valid 0.0; Min. NaN; Mean NaN; StDev NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

X

f5972 Location:

Summary Statistics: Min. NaN; Valid 0.0; Max. NaN; Mean NaN; StDev NaN

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

Y

f5972 Location:

Summary Statistics: Max. NaN; Mean NaN; Min. NaN; Valid 0.0; StDev NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

Z

f5972 Location:

Summary Statistics: StDev NaN; Valid 0.0; Min. NaN; Mean NaN; Max. NaN;

Variable Format: numeric

Notes: UNF:6:6W9cQ9bpMTWkc7U47ZEHLg==

CellviabilityupontreatmentwithHFnandHFnFAPtoexcludeanyactiveroleofFAPtargetingwithfunctionalizednanovectorsincubatedinHMfsfor24h

f5967 Location:

Summary Statistics: Valid 0.0; StDev NaN; Mean NaN; Max. NaN; Min. NaN

Variable Format: numeric

Notes: UNF:6:yQIyWGuAH5VYp28vlj7M2A==

B

f5967 Location:

Variable Format: character

Notes: UNF:6:sGYwLScIsqC185eJhAQRSQ==

C

f5967 Location:

Variable Format: character

Notes: UNF:6:dDCQXelvx/qXLGdWs4885Q==

D

f5967 Location:

Summary Statistics: Min. NaN; Max. NaN; StDev NaN; Mean NaN; Valid 0.0

Variable Format: numeric

Notes: UNF:6:yQIyWGuAH5VYp28vlj7M2A==

E

f5967 Location:

Variable Format: character

Notes: UNF:6:Mrs7ewyv1/gyUncCLf4FBQ==

F

f5967 Location:

Variable Format: character

Notes: UNF:6:J9ZtxkRNB6DcSGo3jJMQBA==

A

f5969 Location:

Summary Statistics: StDev NaN; Max. NaN; Mean NaN; Min. NaN; Valid 0.0

Variable Format: numeric

Notes: UNF:6:YNqjpffb+iAPjIKEDs9bQg==

CellviabilityupontreatmentwithNav,HnavandHNav-FAPfor24h

f5969 Location:

Variable Format: character

Notes: UNF:6:HkIy8oe5SGl+jgJDoW8mFw==

C

f5969 Location:

Variable Format: character

Notes: UNF:6:L85UGXL5l4KVkOKoRBw9qw==

D

f5969 Location:

Summary Statistics: Valid 4.0; Mean 110.44275499999999; StDev 17.35380214735184; Min. 89.14822; Max. 128.128

Variable Format: numeric

Notes: UNF:6:AcsuxeESKdsD4MVPQva3gg==

E

f5969 Location:

Summary Statistics: Valid 4.0; Max. 130.5101; Min. 90.44755; StDev 19.68729018141632; Mean 112.1571125

Variable Format: numeric

Notes: UNF:6:1ScUhzjRd9tQhqRfuDOz9A==

F

f5969 Location:

Summary Statistics: Min. 91.38595; Valid 4.0; Mean 109.7930625; StDev 15.805127479575265; Max. 123.436;

Variable Format: numeric

Notes: UNF:6:ew+XHeksmCsaC2lIWpyCYw==

G

f5969 Location:

Summary Statistics: Max. 122.0645; Valid 4.0; StDev 17.780931424029085; Min. 83.95091; Mean 106.40037749999999

Variable Format: numeric

Notes: UNF:6:QLovpmmVh7wGBI4VpzhC5Q==

H

f5969 Location:

Summary Statistics: Valid 4.0; Max. 113.4023; StDev 12.590720801850791; Mean 102.0512425; Min. 86.33301

Variable Format: numeric

Notes: UNF:6:yz1ApqlBat55s524lFhjSA==

I

f5969 Location:

Variable Format: character

Notes: UNF:6:unIgZbgitFTlRV5pX6oESA==

J

f5969 Location:

Summary Statistics: StDev 11.509889644525998; Min. 105.1732; Mean 121.017775; Valid 4.0; Max. 132.026;

Variable Format: numeric

Notes: UNF:6:P9+z3rUYifi+zhFSrcOH5g==

K

f5969 Location:

Summary Statistics: Max. 133.975; Mean 119.068815; StDev 16.769829922281062; Min. 95.13956; Valid 4.0

Variable Format: numeric

Notes: UNF:6:YDikeiYlymzQ+ZOZPLoofA==

L

f5969 Location:

Summary Statistics: StDev 20.167788457130506; Max. 127.6227; Valid 4.0; Min. 85.25024; Mean 114.990385

Variable Format: numeric

Notes: UNF:6:UGwGFUm76xwNZtH8fSXYPw==

M

f5969 Location:

Summary Statistics: Min. 81.92974; Max. 123.1473; StDev 19.43184426150968; Valid 4.0; Mean 110.316435

Variable Format: numeric

Notes: UNF:6:lttUip6bywAjkbUorPV3wQ==

N

f5969 Location:

Summary Statistics: Max. 121.8479; Valid 4.0; StDev 16.71555344271516; Mean 109.05318; Min. 85.82772

Variable Format: numeric

Notes: UNF:6:o46cKZWMjKvkx9al6ffVYw==

O

f5969 Location:

Variable Format: character

Notes: UNF:6:6VHcS9lbNd0P4x3/d+/0Hw==

P

f5969 Location:

Summary Statistics: Max. 124.5188; Mean 109.9013625; Valid 4.0; StDev 18.19481901610891; Min. 84.74495

Variable Format: numeric

Notes: UNF:6:yiMR0zs16hDjbt36SDz4gA==

Q

f5969 Location:

Summary Statistics: StDev 21.092385065079764; Valid 4.0; Mean 114.55728; Min. 83.44562; Max. 130.1492

Variable Format: numeric

Notes: UNF:6:APUtS1oqjaiidfYgTe/4zw==

R

f5969 Location:

Summary Statistics: Valid 4.0; Min. 84.52839; Max. 135.2743; Mean 115.0445225; StDev 21.644321557320566;

Variable Format: numeric

Notes: UNF:6:rh7PMX9UGvjptLYJlBaFmw==

S

f5969 Location:

Summary Statistics: Min. 76.94899; Max. 124.9519; Valid 4.0; Mean 107.2304975; StDev 21.098425878803337

Variable Format: numeric

Notes: UNF:6:RwKxjIgZD8dCsHjuxHf42g==

T

f5969 Location:

Summary Statistics: Max. 103.1521; Mean 91.31378000000001; StDev 13.078158848431231; Min. 77.45428; Valid 4.0

Variable Format: numeric

Notes: UNF:6:RP5Kh9lQsnqhjL2Y54ItlA==

CellularuptakeofNav,HnavandHnav-FAPincubatedfor1hinHMfsandMDA-MB-231quantifiedbyUPLCMS/MS

f5966 Location:

Variable Format: character

Notes: UNF:6:XN1WCXrRmc0A7ws926RPVw==

B

f5966 Location:

Variable Format: character

Notes: UNF:6:FifVUibDj0Tga7oMbBtakQ==

C

f5966 Location:

Variable Format: character

Notes: UNF:6:xLtvIHrwnBiZrNdc3NtJHA==

D

f5966 Location:

Variable Format: character

Notes: UNF:6:LOkj9hmUjU1GFr9VoeZUAA==

E

f5966 Location:

Summary Statistics: Max. NaN; StDev NaN; Valid 0.0; Mean NaN; Min. NaN;

Variable Format: numeric

Notes: UNF:6:lMEe08PHMBatuSQWNSZ44Q==

F

f5966 Location:

Summary Statistics: Mean NaN; StDev NaN; Max. NaN; Min. NaN; Valid 0.0;

Variable Format: numeric

Notes: UNF:6:lMEe08PHMBatuSQWNSZ44Q==

G

f5966 Location:

Summary Statistics: Mean NaN; StDev NaN; Valid 0.0; Max. NaN; Min. NaN;

Variable Format: numeric

Notes: UNF:6:lMEe08PHMBatuSQWNSZ44Q==

H

f5966 Location:

Summary Statistics: Mean NaN; Max. NaN; Valid 0.0; Min. NaN; StDev NaN

Variable Format: numeric

Notes: UNF:6:lMEe08PHMBatuSQWNSZ44Q==

I

f5966 Location:

Summary Statistics: Valid 0.0; Max. NaN; StDev NaN; Mean NaN; Min. NaN

Variable Format: numeric

Notes: UNF:6:lMEe08PHMBatuSQWNSZ44Q==