10.13130/RD_UNIMI/SFSEFZGuglielmetti, SimoneSimoneGuglielmetti0000-0002-8673-8190DeFENS, Università degli Studi di MilanoReplication Data for 16S rRNA gene sequencing for: "Modulation of Pulmonary Microbiota by Antibiotic or Probiotic Aerosol Therapy: A Strategy to Promote Immunosurveillance against Lung Metastases"UNIMI Dataverse2019Medicine, Health and Life SciencesGuglielmetti, SimoneSimoneGuglielmettiDeFENS, Università degli Studi di Milano2018-07-232023-12-1410.1016/j.celrep.2018.08.09015254137927451727664315447754197905011259991516051311117808991506209599257181242138713290010173368421490904118847937107170371362870714731615181848411027743313191493application/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzipapplication/x-gzip2.1CC0 1.0This dataset contains the following files:\r\n\r\n(1) 20 files \"fastq.gz\": files containing the raw sequencing data (FASTQ files) of the 16S rRNA gene profiling carried out on DNA extracted from BAL of antibiotic-treated (files from A1 to A5) and untreated mice (files from C1 to C5). The 16S rRNA gene was amplified using primers EUBF 5’-TCCTACGGGAGGCAGCAGT-3’ and EUBR 5’ -GGACTACCAGGGTATCTAATCCTGTT-3’ (DOI: 10.1099/00221287-148-1-257), which target the V3-V4 hypervariable regions, and sequenced using MiSeq Illumina® technology (2 x 300 paired-end MiSeq kit V3, set to encompass 467-bp amplicon) as previously described (DOI: 10.1371/journal.pone.0142334; DOI: 10.1111/trf.13477).\r\n\r\n(2) File named \"16S rRNA gene sequences.docx\": Word file containing the nucleotide sequences of part of the 16S rRNA gene amplified from the DNA of the bacterial strains isolated from the bronchoalveolar lavage fluid (BAL) of antibiotic-treated and untreated mice. Before each sequence, the first output of BLASTN search performed with default parameters against the \"16 ribosomal RNA sequences (Bacteria and Archaea)\" database.\r\n\r\nFor any additional need to this datasets, contact: simone.guglielmetti@unimib.it and lucia.sfondrini@unimi.it.