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Project SENSE (Università degli Studi di Milano)
Phyto-rhyzo-remediation is a promising sustainable technology for pollutant clean-up provided by the plant holobiont, composed by the host plant and its microbiota. Plant root exudation is modulated by the pollution stress and has a key role in the activation of the microbial degrading metabolism. Despite the well documented role of the plant holobiont in ecosystem services, the complex interactions between host and microbiome are poorly understood, in particular in contaminated environments. The project will span metabolomics, biosensors and an innovative application of microsensor/sensor devices to profile the chemistry of the root microenvironments and the plant-triggered activation of degrading bacteria. The study will be applied to the site of Brescia-Caffaro, one of the largest sites in Europe contaminated by polychlorinated biphenyls (PCBs). The project aims to sort out the temporal and spatial synergistic interplay within the plant holobiont components and the geochemistry of rhizosphere micro-niches supporting microbial degradation. The project outcomes will provide a comprehensive understanding of the plant holobiont applied to environmental biotechnology, focusing on the soil microbiome degradative potential.
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1 to 4 of 4 Results
Jun 14, 2021
The objective of WP4 is to investigate the portioning of aerobic and anaerobic niches in PCB contaminated environments through microsensor devices and a fluorescent sensor imaging system. The data will be generated through approaches in plant physiology for plant growth under dif...
Jun 14, 2021
The objective of WP3 is to investigate the plant-modulated chemical micro-habitats in the rhizosphere and endosphere through microsensor devices during plant-microbe interaction in a PCB contaminated environment. The data will be generated through approaches in plant physiology f...
Jun 14, 2021
The objective of WP2 is to generate a bacterial biosensor to monitor the dynamics of activation of PCB degradation pathways in bacteria upon stimulation by plant root exudates. The data will be generated through approaches of molecular biology to generate the fusion between the p...
Jun 14, 2021
The objective of WP1 is to investigate potential plant modulators exudated by Arabidopsis thaliana roots affecting bacterial degradation of polychlorinated biphenyls (PCBs). The experimental data will combine plant physiology to the set-up of a protocol for root exudates collecti...
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